Journal: Parasites & Vectors

Article Title: Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis

doi: 10.1186/1756-3305-5-30

Figure Lengend Snippet: Scatter plots demonstrating the degree of variance between different sample hybridisations . Scatter plots were constructed from the raw non-normalised, transformed (natural log) array data, averaged for each comparison from the four hybridisations (2 dye-swapped (Cy3/Cy5) replicates each). A . Self vs self hybridisation (Fed P. ovis control vs fed P. ovis control). B . Biological replicates (replicated analysis of fed P. ovis control vs starved P. ovis , compared using two separate dye swapped arrays). C . Activated sample vs control sample (starved P. ovis vs fed P. ovis control). Scatter plots, linear regression analysis and corresponding R 2 values were generated in GraphPad Prism (GraphPad Software Inc, Version 5.04).

Article Snippet: Hybridisations were performed on a MAUI microarray hybridisation system (BioMicro Systems, USA) using the MAUI Mixer A4 hybridisation cassette with a 16 μl fill volume per chamber (BioMicro Systems, USA) which allowed the four microarrays on a single slide to be treated independently.

Techniques: Construct, Transformation Assay, Hybridization, Generated, Software

Journal: Parasites & Vectors

Article Title: Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis

doi: 10.1186/1756-3305-5-30

Figure Lengend Snippet: Scatter plots demonstrating the degree of variance between different sample hybridisations . Scatter plots were constructed from the raw non-normalised, transformed (natural log) array data, averaged for each comparison from the four hybridisations (2 dye-swapped (Cy3/Cy5) replicates each). A . Self vs self hybridisation (Fed P. ovis control vs fed P. ovis control). B . Biological replicates (replicated analysis of fed P. ovis control vs starved P. ovis , compared using two separate dye swapped arrays). C . Activated sample vs control sample (starved P. ovis vs fed P. ovis control). Scatter plots, linear regression analysis and corresponding R 2 values were generated in GraphPad Prism (GraphPad Software Inc, Version 5.04).

Article Snippet: Hybridisations were performed on a MAUI microarray hybridisation system (BioMicro Systems, USA) using the MAUI Mixer A4 hybridisation cassette with a 16 μl fill volume per chamber (BioMicro Systems, USA) which allowed the four microarrays on a single slide to be treated independently.

Techniques: Construct, Transformation Assay, Comparison, Hybridization, Control, Generated, Software

Journal: Ecology and Evolution

Article Title: Population genomics of resource exploitation: insights from gene expression profiles of two Daphnia ecotypes fed alternate resources

doi: 10.1002/ece3.30

Figure Lengend Snippet: Gene symbols, microarray probe identifiers, and expression levels in the microarray experiment for genes that differed significantly between treatments in at least one ecotype. A is the average fluorescence level across treatments. A -values for spotting-buffer controls were ∼ 4.1. M is the relative expression of the field seston-treated individuals to the controls, in log 2 units. Boldface type indicates significantly different expression between the field seston treatment and the lab food control

Article Snippet: We used MAUI SC mixers, a 4-bay MAUI Hybridization system, and Pronto Universal Microarray Hybridization kits (Corning; Corning, NY, USA) according to the manufacturer recommendations.

Techniques: Microarray, Expressing, Fluorescence